clone ja9  (Novus Biologicals)

Journal: Hormones & cancer

Article Title: MOLECULAR AND ELECTROPHYSIOLOGICAL ANALYSES OF ATP2B4 GENE VARIANTS IN BILATERAL ADRENAL HYPERALDOSTERONISM

doi: 10.1007/s12672-019-00375-0

Figure Lengend Snippet: A) Rare gene variants in ATP2B4 ; B) Clinical characteristics of patients with hereditary or sporadic germline variants in ATP2B4.

Article Snippet: After antigen retrieval in pH 6 citrate buffer and pH 9 Tris buffer respectively, the sections were incubated for 1 h in primary antibodies for CYP11B2 (mouse monoclonal clone 41–17B; 1:100; kindly donated by Dr. Celso Gomez-Sanchez) and PMCA4b (mouse monoclonal clone JA9; Catalog# MA1–914, Millipore; 1:100).

Techniques: Variant Assay

Journal: Hormones & cancer

Article Title: MOLECULAR AND ELECTROPHYSIOLOGICAL ANALYSES OF ATP2B4 GENE VARIANTS IN BILATERAL ADRENAL HYPERALDOSTERONISM

doi: 10.1007/s12672-019-00375-0

Figure Lengend Snippet: Expression of ATP2B4 in tissues. (A) Real time analyses of ATP2B4 transcript variant 2 in adrenal tissues in comparison with various tissues. *p<0.05 versus pancreas (B) Zonal expression of ATP2B4 mRNA across the human adrenal using laser capture microdissection. The three probes correspond to 205410_s_at, 212136_at, 212135_s_at (top to bottom), which target the 3’UTR of both ATP2B4 transcripts. (C) Immunofluorescence for PMCA4b protein on serial sections of the human adrenal cortex: (i) H&E (ii) negative control incubated only with secondary antibody and DAPI (iii, iv) expression of CYP11B2 and PMCA4b, respectively.

Article Snippet: After antigen retrieval in pH 6 citrate buffer and pH 9 Tris buffer respectively, the sections were incubated for 1 h in primary antibodies for CYP11B2 (mouse monoclonal clone 41–17B; 1:100; kindly donated by Dr. Celso Gomez-Sanchez) and PMCA4b (mouse monoclonal clone JA9; Catalog# MA1–914, Millipore; 1:100).

Techniques: Expressing, Variant Assay, Comparison, Laser Capture Microdissection, Immunofluorescence, Negative Control, Incubation